Isoquinoline alkaloids are the main group of secondary metabolites present in Chelidonium\nmajus extracts, and they are still the object of interest of many researchers. Therefore, the development\nof methods for the investigation and separation of the alkaloids is still an important task. In this\nwork, the application potential of a silica-based monolithic column for the separation of alkaloids\nwas assessed. The influence of the organic modifier, temperature, salt concentration, and pH of the\neluent on basic chromatographic parameters such as retention, resolution between neighboring peaks,\nchromatographic plate numbers, and peak asymmetry were investigated. Based on the obtained\nresults, a gradient elution program was developed and used to separate and quantitatively determine\nthe main alkaloids in a Chelidonium majus root extract.
Loading....