Herbal preparations from Silybum marianum have been used since the fourth century BC \nin liver disease treatment and against numerous other pathologies. Consumption of silymarin \ncontaining drugs and food supplements continues to increase. Precise, fast, reliable, and complex \ndetermination of all components of silymarin preparations is paramount for assessing its \npharmacological quality. We present here simple and fast HPLC --DAD and LC --MS analytical \nmethods for the determination and quantification of all known silymarin components, including \n2,3 --dehydroflavonolignans that has not been achieved so far. The first method, using a common C18 \ncolumn, allows baseline separation of previously inseparable silychristin A, B, isosilychristin, and \nsilydianin. Moreover, this method allowed detection of three so far unknown silymarin \ncomponents.
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