Mefenamic acid (MEFE) and Dicyclomine hydrochloride (DICYCLO) are used in combined dosage form for the treatment of irritable bowel syndrome (IBS), Inflammation in abdominal pain and muscular spasm. Literature describes, no method has been reported for the simultaneous estimation of MEFE and DICYCLO in combined dosage form by UV spectrophotometry. For the simultaneous estimation of MEFE and DICYCLO by two UV spectrophotometry methods have been developed. The, (1) First order derivative method, and (2) Second order derivative method has been developed. For the linearity for MEFE and DICYCLO are lies between 40-80 µg/mL and 1.6-3.2 µg/mL respectively with co-relation co-efficient > 0.998. The solvent is used is 0.1N NaoH. For the first order derivative method the wavelength selected are 274.59 nm for MEFE and 252.95 nm for DICYCLO, for the second order derivative method the wavelength selected are 259.5 nm for MEFE and 271.55 nm for DICYCLO. All the developed methods are validated according to ICH guidelines. The proposed methods for estimation of MEFE and DICYCLO were found to be simple, precise, accurate, economical and statistically validated and are applicable for the simultaneous determination of MEFE and DICYCLO in ophthalmic dosage form.
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