A new, simple, and reliable UV-spectrophotometric absorption correction method has been developed for simultaneous determination of Piperacillin and Tazobactam in combined pharmaceutical dosage form. The method is based upon determination of Piperacillin at 270 nm and Tazobactam at 225 nm, in aqueous solution. Piperacillin and Tazobactam show linearity in the concentration range of 10-80 μg/ml and 2-12 μg/ml respectively. The percentage recovery was in the range of 99.92% ‐ 100.27% for Piperacillin and 99.88% ‐ 100.30% for Tazobactam. The developed method was validated statistically and the % RSD value was found to be less than 2.
Loading....