A Reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous estimation of ezetimibe (EZT) and simvastatin (SMV) in marketed tablet formulation .The determination was carried out on Inertsil ODS-3V, (150 × 4.6 mm, 5µ) column and 0.1% orthophosphoric acid in Milli Q water adjust PH 4.2 with triethylamine, methanol and acetonitrile (45:15:40) used as a mobile phase in gradient technique, at a flow rate of 1.8ml/min, the detection was carried out at 238nm. Retention time of EZT and SMV were found to be 5 and 10.50 min, respectively. The method has been validated according to ICH Q2 (R1) guidelines. Linearity for EZT and SMV were found in the range of 5ppm-15ppm for both drugs. The correlation coefficient (r2) for EZT and SMV were found to be 0.9999 and 0.9998, respectively. The mean recoveries obtained for EZT and SMV were found to be 101% and 101.5%, respectively .Developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of EZT and SMV in tablets.
Loading....