Three simple, sensitive, rapid and accurate colorimetric methods (Method 1, 2 and 3) have been developed for the estimation of Artesunate in bulk and pharmaceutical dosage forms. Method 1 was based on the formation of green colored complex between Artesunate and 3- methyl-2 benzothiazolinone hydrazone hydrocholoride (MBTH) in presence of ferric chloride, which shows maximum absorbance at 661nm. Method 2 was based on the formation of pink coloured complex formation between Artesunate and 3, 5 –dinitrosalicyliacid (DNSA) in alkaline condition, which shows maximum absorbance at 510nm. Method 3 was based on the formation of yellowish coloured complex formation between Artesunate and P – dimethylaminobenzaldehyde (PDAB) in acidic condition, which shows maximum absorbance at 468nm. The linearity was found to be 10-50, 50-250 and 15-75 μg/ml for method 1, 2 and 3 respectively. Proposed methods were validated statistically and the recovery studies were carried out by standard addition method.
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