Method development is the process of determining what conditions are adequate and/or ideal for the analysis required. In Gas chromatography(GC) carrier gas is sometimes selected based on the sample''s matrix, for example, when analyzing a mixture in argon, an argon carrier is preferred, Which gas to use is usually determined by the detector. Many modern GCs, however, electronically measure the flow rate, and electronically control the carrier gas pressure to set the flow rate. Consequently, carrier pressures and flow rates can be adjusted during the run, creating pressure/flow programs similar to temperature programs. The polarity of the solute is crucial for the choice of stationary compound; in GC both open as well as packed column are used. In GC injection technique depends on state of sample that is either in liquid, gas adsorbed or solid form. Sample injection system is based on differences in boiling point, polarity, concentration or thermal/catalytic stability and column selection depend upon polarity of sample but mostly depends upon functional group of sample. Column temperature is selected to compromise between the length of the analysis and the level of separation. The thermal-conductivity (TCD) and flame-ionization (FID) detectors are the two most common detectors on commercial gas chromatographs. Method validation parameter are depends on the stage of product readiness at the point of submission for approval. The objective of validation of an analytical procedure is to demonstrate that it is suitable for its intended purpose.
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