The aim of this present work was to develop and validate a simple, rapid, accurate RP-HPLC method for the simultaneous estimation of Alfuzosin hydrochloride (ALH) and Dutasteride (DUT) in bulk drug and pharmaceutical dosage form. The chromatographic separation was carried out in an isocratic mode using an Kromasil C18 column (150mm×4.6mm,5µm particle size) with a mobile phase of mixed phosphate buffer:acetonitrile(30:70%v/v) and the eluents were monitored at 242nm.The retention times of ALH and DUT were 2.2 and 7.2min respectively. The method was found to be linear over the concentration range of 5-30µg/ml for ALH and 0.25-1.5µg/ml for DUT with a correlation coefficient of 0.999. The proposed RP-HPLC method was validated according to ICH guidelines and was successfully employed for routine quality control analysis in bulk and combined dosage forms.
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