A simple, fast, and validated HPLC method was developed for the simultaneous quantization of five cardiovascular agents: dopamine
(DPM), dobutamine (DBM), phentolamine (PTM), furosemide (FSM), and aminophylline (APL) either in infusion samples or in an
injection dosage form. *e proposed method was achieved with a 150mm× 4.6 mm, 5.0 μm C18 column, by using a simple linear
gradient. Mobile phase A was buffer (50mMKH2PO4) and mobile Phase B was acetonitrile at a flow rate of 1.0 mL/min. *e column
temperature was kept at 30°C, and the injection volume was 20 μL. All analytes were separated simultaneously at a retention time (tr)
of 3.93, 5.84, 7.06, 8.76, and 9.67 min for DPM, DBM, PTM, FSM, and APL, respectively, with a total run time of less than 15.0 min.
*eproposed method was validated according to ICH guidelines with respect to accuracy, precision, linearity, limit of detection, limit
of quantitation, and robustness. Linearity was obtained over a concentration range of 12.0–240.0, 12.0–240.0, 20.0–200.0, 6.0–240.0,
and 10.0–200.0 μg/mL DPM, DBM, PTM, FSM, and APL, respectively. Interday and intraday accuracy and precision data were
recorded in the acceptable limits. *e new method has successfully been applied for quantification of all five drugs in their injection
dosage form, infusion samples, and for evaluation of the stability of investigated drugs in mixtures for endovenous use. *e results of
the stability study showed that mixtures of DPM, DBM, PTM, FSM, and APL in 5% glucose or 0.9% sodium chloride injection were
stable for 48 hours when stored in polypropylene syringes at 25°C.
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