A simple, precise and rapid high-performance thin layer chromatography method is developed and validated for analysis of tizanidine in plasma. Following liquid-liquid extraction, the analytes are separated using toluene: acetone: ammonia (6:6:0.4 v/v/v) as a mobile phase on Merck HPTLC aluminium backed sheets of silica gel 60 F254 and scanned at 254 nm using densitometer. The linearity of tizanidine (r2=0.991±0.001) in plasma is found in range of 300–1100 ng/spot. Standard curve concentration range shows acceptable precision and accuracy. Method is validated according to I.C.H. and U.S.F.D.A. guidelines. Intraday and interday precision is less than 15 % R.S.D. while average % recovery is found to be 99.72 with average % R.S.D. 0.4865. Statistical analysis suggests that the method is repeatable and selective for estimation of tizanidine in plasma. This method could effectively analyze tizanidine in plasma hence can be employed for application in pharmacokinetic, bioavailability or bioequivalence studies.
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