The present research work aims to develop a simple, sensitive, accurate and reproducible method for the simultaneous estimation of Avanafil and Dapoxetine hydrochloride by two different spectroscopic method viz, Simultaneous equation Method (Vierodt's method) (Method-A) and Q-Absorption method (Method-B). Absorbance maxima for Avanafil and Dapoxetine hydrochloride were found to be at 247 nm and 211 nm respectively for method-A, and Iso-absorptive point for both drugs was found to be 226.6 nm for method-B by using 0.1 N HCl as a solvent. Absorption maxima of avanafil at 247nm, was taken as second wavelength for determination by method-B. Linearity for both the methods was observed in concentration range of 2.5-15 μg/ml for Avanafil and 1.5-9 μg/ml for Dapoxetine hydrochloride. The correlation coefficients for both the methods obtained were near to 1. The accuracy of both methods was evaluated by recovery studies and recovery results were obtained from 98 % to 102% for both the methods and the relative standard deviations below 2% were achieved. Validation was done as per ICH guidelines. Proposed methods can estimate Avanafil and Dapoxetine hydrochloride simultaneously in combined dosage form without the interference of common excipients.
Loading....