A binary mixture of Levofloxacin and Ornidazole was determined by RP-HPLC method. The method involved determination of Levofloxacin and Ornidazole by reversed-phase liquid chromatography using water: acetonitrile: triethylamine (75:25:0.1, v/v) and final pH adjusted to 3.15 ± 0.02 with 5% v/v ortho-phosphoric acid and was pumped at a flow rate of 1.5 ml/min. Quantification was achieved with ultraviolet detection at 310 nm over concentration ranges of 0.5-8.0 µg/ml and 1-16 µg/ml; mean accuracies were 98.74 - 100.5 and 98.89 - 102.3%, respectively. The method was successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients were found. The method retained its accuracy and precision when the standard addition technique was applied.
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