Gene is a building block of body and through knowledge about gene and genetically derived products is called as Genetic engineering. Genetic Engineering is a part of Biotechnology. Biotechnology has been defined as the application of biological organisms, system or processes to manufacturing and service industries. Genetic Engineering is defined as genetic modification; it is the direct manipulation of any organisms, genome using modern DNA technology. Biotechnology having wide application in field of Pharmaceutical, Agriculture Industry, Beverages, Dairy products, Environmental science, Medical & Health sciences, Amino acid production. Both Qualitative and quantitative methods are now available for identification and quantification of gene. Various analytical methods like Protein Content Analysis, Amino Acid Analysis, Protein sequence, Peptide Mapping, Immunoassay, Electrophoresis, Chromatography, DNA determinations, Quantitative Assay, Biomimetic Assay, Carbohydrate determination, Adventitious and endogenous protein detections used for identification of gene and genetic materials. Other methods like Uv spectrophotometry, Kjeldahl nitrogen analysis, Biuret assay, Lowry protein, Bradford assay, Bicinchoninic acid assay, Fluoroscent methods used for protein analysis. Protein content assays are among the most important of all the methods used for biotechnology derived products because the results of other types of assays, such as potency, are also dependent on them.
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