The tubers of Dicentra paucinervia, a highly potent ethno medicinal herb, have been used by tribals living in the eastern Nagaland and adjoining Manipur states of India in the treatment against various diseases. The plant was also grown in Dehradun to examine its adaptability and utilization in the Uttarakhand state. Allocryptopine was one of the major alkaloid constituents found in the tubers of the plant. The content of allocryptopine was determined in the tubers of the plants grown in Manipur and Dehradun. HPTLC method was first validated using pure allocryptopine and applied for its quantittaion in the tubers. The method validation was comprised of (i) chromatographic conditions, (ii) linearity, (iii) precision, and (iv) recovery experiments. The method was found to be simple, rapid and accurate for quantification of allocryptopine in the tubers of Dicentra paucinervia. The allocryptopine content was determined to be 0.69% (Manipur) and 0.70% (Dehradun).
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