A simple and precise High-performance thin-layer chromatographic (HPTLC) method for determination of amlodipine besylate (AMB) and perindopril erbumine (PER) in their bulk and tablet dosage forms has been developed and validated. This employs a precoated silica gel 60F254 on aluminium sheets and mobile phase was Chloroform: Ethanol: Toluene: Glacial acetic acid in the ratio of 5:3:3.5:0.5v/v. having chamber saturation for 20 minutes at room temperature. The TLC plate was run up to 8 cm. Detection was performed at 230 nm. The RF values were 0.15 for amlodipine and 0.85 for perindopril. The developed method validated for its accuracy and precision. The LOD and LOQ were found to be 0.1 μg/ml and 0.3 μg/ml, 0.1 μg/ml and 0.3 μg/ml respectively for amlodipine and perindopril. The recovery was carried out by standard addition method. The Average recovery was found to be 99.22% and 100.73% for amlodipine and perindopril respectively.
Loading....