A simple and sensitive spectrophotometric method has been developed for simultaneous determination of Betaxolol Hydrochloride and Chlorthalidone in a binary mixture. In the proposed method, the absorbance was measured at 282.0 nm and 250.0 nm corresponding to the absorbance maxima of Betaxolol and Chlorthalidone in methanol, respectively. Linearity range was observed in the concentration range of 4-14 μg/ml for Betaxolol HCl and 10-60 μg/ml for Chlorthalidone. Concentration of each drug was obtained by using the absorptivity values calculated for both drugs at two wavelengths, 282.0 nm and 250.0 nm and solving the simultaneous equation. Developed method was applied to laboratory mixture. The method was validated statistically and recovery study was performed to confirm the accuracy of the method. The method was found to be rapid, simple, accurate and precise.
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