Atazanavir sulphate (ATV) is a new HIV-1 protease inhibitor anti retroviral drug. The present study describes a simple, accurate, reproducible and precise UV Spectrophotometric method for the estimation of ATV in 0.025 N HCl. ATV is insoluble in 0.025 N HCl, so methanol was used as a cosolvent. The absorbance maximum (λmax) was found to be 300 nm with linearity in the range of 5-60µg/ml and regression equation as Y = 0.013-0.001. Regression coefficient was calculated as 0.999. The method was validated for different parameters such as sandell’s sensitivity, molar absorptivity, accuracy, precision, ruggedness, robustness, detection limit, quantification limit, etc (as per the ICH guidelines). Molar absorption coefficient and sandell’s sensitivity comes out to be 1.06 X 104 l.mol-1cm-1 and 0.1538µg/ml/0.001 A.U. respectively. LOD and LOQ were found to be 0.2071µg/ml and 0.627µg/ml respectively. The relative standard deviation (RSD) in case of accuracy, precision, ruggedness and robustness was less than 2.0% proving that method was highly accurate, precise and robust. This method can be used for the determination of ATV in pharmaceutical formulations without interference of the excipients.
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