A selective and sensitive spectrophotometric method for determination of Granisetron has been developed and validated by three simple, precise and economical UV spectrophotometric methods have been developed for the estimation of Granisetron in bulk and tablet dosage form. A review of the literature revealed no methods for determination of granisetron in pharmaceutical dosage forms by UV spectrophotometric methods and previously reported methods were mainly focused on the analysis of this drug in biological fluids which includes HPLC methods with fluorescence detection (1-4) and LC-MS/MS methods(5-6) for determination of granisetron in human biological fluids. The BP(7) describes an HPLC method for the determination of Granisetron in bulk i.e. raw material. Since the UV spectrophotometric methods are simple and fast for routine analysis. Granisetron has the absorbance maxima at 302 nm (Method A), and in the first order derivative spectra showed sharp peak at 291 nm (Method B). Method C applied was in the wavelength range of 298-312 nm. Linearity for detector response was observed in the concentration range of 1-12μg/mL for Method A, Method B and Method C. This method was validated according to validation procedures, parameters and acceptance criteria based on USP XXIII guidelines and recommendations of Shah et al.(8-9). The proposed methods were successfully applied for the simultaneous determination of Granisetron in commercial tablet preparation.
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