Two simple, rapid, precise and accurate spectrophotometric methods have been developed for simultaneous analysis of Cinitapride Hydrogen Tartrate (CNT) and Rabeprazole Sodium (RAB) in their combined dosage form. Method A, Ratio derivative spectrophotometry, involves division of spectra of Cinitapride Hydrogen Tartrate by one selected standard spectrum of Rabeprazole sodium and then measuring absorbance at 277.70 nm in ratio derivative spectra for estimation of Cinitapride Hydrogen Tartrate. Similarly, spectra of Rabeprazole Sodium are divided by one selected standard spectrum of Cinitapride Hydrogen Tartrate and then measuring absorbance at 281.40 nm in ratio derivative spectra for estimation of Rabeprazole Sodium. Method B, 1st Derivative Q-method, It involves formation of Q-absorbance equation at 289.80 nm (isoabsorptive point) and 255.80 nm (λ max of Cinitapride Hydrogen Tartrate) in 1st derivative spectra. Developed methods were validated according to ICH guidelines. The calibration graph follows Beer’s law in the range of 4.0 to 20.0 μg/ml for Cinitapride Hydrogen Tartrate and 4.0 to 20.0 μg/ml for Rabeprazole Sodium in Distilled water as a solvent with R square value greater than 0.999. Accuracy of all methods was determined by recovery studies and showed % recovery between 98 to 102 %. Intraday and inter day precision was checked for all methods and mean %RSD was found to be less than 2 for all the methods. The methods were successfully applied for estimation of Cinitapride Hydrogen Tartrate and Rabeprazole Sodium in marketed formulation.
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