A sensitive, selective, precise and stability indicating high performance thin layer chromatographic method of analysis of valacyclovir hydrochloride in bulk drug and in formulations was developed and validated in pharmaceutical dosage form. The method employed TLC aluminium plates precoated with silica gel 60F-254 as stationary phase. The solvent system consisted of toluene: methanol: diethylamine (8:1:1 v/v/v). This system was found to give compact spots for Valacyclovir hydrochloride (retention factor value of 0.39). Densitometric analysis of valacyclovir hydrochloride was carried out in the absorbance mode at 252 nm. The linear regression data for the calibration plots showed good linear relationship with r2=/0.997/in the concentration range of 100-500 ng/band. The method was validated for precision, accuracy, robustness and specificity. The limits of detection and limits of quantitation were 33.3 ng/spot and 100 ng/spot respectively. The drug undergoes acidic, alkaline, neutral, oxidation, dry heat and photo degradation treatment. Statistical analysis proved the method is an economic, reproducible, accurate and selective for estimation of valacyclovir hydrochloride. Because the method could effectively separate the drug from its degradation products, it can be employed as a stability indicating method.
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