The present research work discusses the development of a UV spectrophotometric method for Bromelain. Simple, sensitive, reproducible, accurate and cost efficient spectrophotometric method has been developed for the estimation of Bromelain in bulk. The optimum conditions for the analysis of the drug were established. The study was conducted in phosphate buffer pH 6.8. The maximum wavelength (λmax) was found to be 280nm. The method validation was accomplished through evaluation of analytical parameters of linearity, range, accuracy and precision, limit of detection and limit of quantification and robustness as per ICH guidelines. The developed method was linear (R2= 0.997) in the concentration range of 100-1000 μg/ml, precise (%RSD inter day= 0.68, 0.35, 0.83 for three consecutive days, %RSD intraday= 0.79, 0.23, 1.62), accurate (% recovery= 98.75-99.27%) and sensitive (LOD and LOQ of 3.34 and 10.12 µg/ml respectively). The proposed method is observed to be suitable for the analysis of Bromelain in bulk for quality control purposes.
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