The present work describes a validated reverse phase high performance liquid chromatographic method for simultaneous estimation of Diclofenac sodium and Famotidine in pharmaceutical dosage form. Chromatography was performed on a Varian C18 column (250 mm x 4.6 mm i.d., 5 µm particle size), column with mobile phase containing acetonitrile, methanol and water in the ratio of 25:30:45 v/v.The flow rate was 1.0 ml/min and the eluent was monitored at 283 nm. The selected chromatographic conditions were found to effectively separate Diclofenac sodium (RT- 2.432 min) and Famotidine (RT- 5.082 min). Linearity for Diclofenac sodium and Famotidine were found in the range of 5-35μg/ml and 2-14μg/ml. The values obtained of LODs were 0.036µg/ml and 0.028µg/ml; LOQs were 0.11µg/ml and 0.088µg/ml for Diclofenac sodium and Famotidine, respectively. The proposed method was found to be fast, accurate, precise, reproducible and rugged and can be used for simultaneous analysis of Diclofenac sodium and Famotidine in combined pharmaceutical formulations.
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