The hydro-distilled medicinal fixed oil of the Calotropis gigantea (Asclepiadaceae) commonly called as Rui was investigated by GC-MS. Six compounds from Calotropis gigantea was characterized. Methyl Octadecanoate (Steric acid 55.95%), Hexadecanoic acid (Palmitic acid 30.06%), Tetradecanoic acid (Myristic acid 8.17%) were found to be the major compounds of Calotropis gigantea while Heptadecanoic acid (2.41%), Ethyl linoleate (1.38%),9, 10-Octadecanoic acid (Linoleic acid 4.03%) were found to be minor compounds. The purpose of the present study was to examine the fatty acid composition of seed oil.
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