A simple, sensitive, rapid, accurate, precise and economical first order derivative spectrophotometric method has been developed for the simultaneous determination of Fluticasone Propionate (FLP), Azelastine Hydrochloride (AZH) in combined nasal spray preparations. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectra were obtained in acetonitrile and the determinations were made at 253 nm (ZCP of Azelastine Hydrochloride) for Fluticasone Propionate and 236 nm (ZCP of Fluticasone Propionate) for Azelastine Hydrochloride. The linearity was obtained in the concentration range of 10-30 μg/ml for Fluticasone Propionate and 28-84 μg/ml for Azelastine Hydrochloride. The mean % recovery was 99.96±0.39% and 100.29±0.37% for Fluticasone Propionate and Azelastine Hydrochloride, respectively. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method.
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