The quality of the herbals depends on the type of extraction procedures used. In the present study targeted analytical plant marker Embelin which has Proven therapeutic effect was extracted using soxhlet apparatus and percolation method from Embelia ribes plant seeds commonly called as vidang. The isolated compound has similar identical characteristics when compared with standard. The % purity and Quantitative analysis of Embelin in raw material and marketed churna was done by developed validated chromatographic methods. The purity of the compound extracted by soxhlet and percolation procedures was found to be 98.02% and 100.47%, respectively. The linearity range for HPLC and HPTLC was found to be 50-100 µg/mL and 200-1200 ng/spot, respectively. HPLC analyses were carried out at using Kromasil C18 column (5 μm, 4.6 × 150 mm). The mobile phases consisted of acetonitrile (A) and phosphate buffer, pH 3.0 (B) in proportion of 95:5 v/v carried out at 280 nm and for HPTLC the mobile phase consisted of n-hexane: ethyl acetate: formic acid in the proportion of 5: 4: 1% v/v/v. scanned at 292 nm .Satisfactory validation parameters results for HPLC were obtained with respect to repeatability %RSD is 1.37, and recovery (98.93-100.36% recovery). In case of HPTLC; repeatability % RSD is 1.89 and % recovery is 98.97-101.77, respectively. Hence the developed validated method can be used for the routine quality control of raw material variability and inconsistency of its content in finished products and these methods can also be useful to determine the %purity for in house isolated Embelin standard.
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