A rapid ultra-performance liquid chromatography (UPLC) method was developed for feasible separation and quantification of 5%w/v solution of multiple amino acids for intravenous infusion. The analysis was performed on Accquity UPLC system with Accquity UPLC AccQ. Tag Ultra Column within 8 min. The correlation coefficient values (r2 > 0.9978) indicated good correlations between the compounds’ concentrations and their peak areas within the test ranges. The limits of quantitation and detection of 18 amino acids were 30–198 ng/mL and 10–70 ng/mL, respectively. The recoveries ranged from 99.84% to 103.89% and the overall relative standard deviations for intra and inter day were lower than 1.4%. The results showed that UPLC was a powerful tool for the analysis of amino acids.
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