Artesunate and Mefloquine is Antimalarial drug belonging to class Sesquiterpine lactones and Quinoline-methanol for the treatment of uncomplicated malaria. Only RP-HPLC, UV spectrophotometric and HPTLC method was reported for the estimation of Artesunate and Mefloquine hydrochloride either alone or in any other drug combination .There was no reported stability indicating HPLC method for the estimation of Artesunate and Mefloquine Hydrochloride. So, purpose of the present work is to a develop and validate simple, precise and stability indicating RP-HPLC method for simultaneous estimation of Artesunate and Mefloquine Hydrochloride in tablet dosage form according to ICH guidelines and to communicate here rapid and cost-effective quality-control tool for routine qualitative analysis of two drugs their combined dosage forms by Chromatographic Methods. RP-HPLC method for the estimation of Artesunate and Mefloquine Hydrochloride was developed by using C18 (250mm X 4.6mm, 5µm) as a stationary phase and Acetronitrile: Phosphate Buffer adjusted with triethylamine (70:30 v/v), pH 3.5 as a mobile phase at 225 nm. RP-HPLC method retention time for Artesunate and Mefloquine Hydrochloride was found to be 3.13 and 4.15 with a linearity range of 50-150µg/ml (R2 =0.997) and 110-330µg/ml (R2 =0.997). Recovery found was found to be 98-101% for Artesunate and 99.3- 101.3% for Mefloquine Hydrochloride. The Stability indicating method by RP-HPLC were found to be accurate, precise, specific, simple and stability indicating. Artesunate and Mefloquine hydrochloride show degradation in acidic, alkaline, oxidative and thermal condition.
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