A fixed dose combination of Cefixime Trihydrate (CEF) and Azithromycin Dihydrate (AZI) is used in ratio of 2:2.5 as tablet for the treatment of the respiratory tract infections, typhoid and pneummonia. A simple, precise and accurate HPTLC method was developed and validated for determination of CEF & AZI in tablet. The compounds were separated on aluminium-backed silica gel 60 F254 plates with Toluene: Chloroform: Methanol: Ammonia (4: 2: 12: 0.2 v/v/v/v) as mobile phase. These systems were found to give sharp spots of CEF and AZI with the Rf value of 0.76 �±0.02 and 0.26 �±0.02 respectively. Densitometric analysis of CEF and AZI were done at 289 nm and 483 nm, respectively. AZI spot is visualized by using Sulfuric Acid : Ethanol (1: 4 v/v) & heated at 120 �°C for 5 min. Method was found linear in the range of 250-1500 ng/band for CEF and 312.5-1875 ng/band for AZI with the correlation coefficient (r2) 0.998 for both drugs. The method was validated for linearity, precision, accuracy, limit of detection (LOD) and limit of quantitation (LOQ). Analysis proved that method is Reproducible, precise, selective, and accurate for simultaneous estimation of CEF and AZI.
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