New feasible, sensitive, rapid and economic spectrophotometry method has been developed for the simultaneous\r\nquantitative determination of Berberine (BER) and Curcumin (CUR). The plant extract mixtures and polyherbal formulation was\r\nestimated with the help of first derivative zero crossing spectrophotometry method. BER and CUR showed maximum\r\nabsorbance at 307 nm and 428.5 nm and obeyed Beerââ?¬â?¢s law in the concentration range 15-40 ?g/ml and 1-6 ?g/ml respectively.\r\nThe method was validated in terms of linearity (R2=0.997, 0.998), precision in terms of % relative standard deviation (R.S.D.) for\r\nintraday and interday of BER and CUR was within limit (0.184%ââ?¬â??0.617% and 0.141%-0.661% respectively), accuracy (within\r\nthe limit), LOD and LOQ (0.029, 0.047 and 0.089, 0.141) and specificity. The methods were found to be robust to variation in\r\nabsorbance due to variation in wavelength (?max Ã?± 2.0). By this method, C. longa and T. cordifolia showed 4% w/w curcumin and\r\n0.2% w/w berberine respectively in combined methanolic extracts of the two herbal drugs. This method was simple, precise,\r\naccurate, sensitive and reproducible and can be used for the routine quality control testing for the marketed polyherbal\r\nformulations and standardization of both plants (Tinospora cordifolia and Curcuma longa).
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