A simple, precise, accurate, reproducible, robust HPLC method was developed for the estimation of Rizatritan benzoate from oro-dispersible tablet dosage form .The method was validated as per ICH and FDA guidelines. Analysis of the drug was performed on Phenomenex Luna C18 (250mm×4.6 mm, 5µ) column, in an isocratic mode employing pH 6.5 phosphate buffer, Acetonitrile and methanol (87:7.8:5.2) as the mobile phase. UV-Visible detector at 225nm was found to be suitable for detection. Linearity was observed in the range of 5-60 µg/ml. Recovery values were found to be 98.2-100.4% .The % RSD values for method precision and system precision were found to be 0.22 and 0.20 respectively. Limit of detection and limit of quantification of the method were found to be0.0014 µg/ml and 0.0043µg/ml respectively.
Loading....