A rapid, economic, sensitive and reproducible colorimetric method for quantitative estimation of Amikacin from bulk and pharmaceutical formulations has been developed. The method employs 1% cobalt nitrate reagent in presence of 2Molar sodium hydroxide to form violet color complex with Amikacin. The concentration of Amikacin over a range of 1.6-4.8 miligram/mililiter was found to obey Beer-lambert’s law. The developed method was validated in accordance with ICH Q2 guidelines. The mean percentage recovery was found to be 99.88% to 100.09%. The developed method was compared with reported RP-HPLC method. The developed method could be used for routine analysis of Amikacin in bulk and formulation due to simplicity, ease of operation and short time required for quantification.
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