New three high performance liquid chromatography (HPLC) -chemometric combinations were implemented for the simultaneous determination of ibuprofen and famotidine in tablets. Three chemometric calibration techniques; classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS) were applied to the ratio of peak areas at multiwavelength using Diode Array Detector (DAD) responses at 245nm, 250nm, 255nm, 260nm and 265 nm. These combinations were called HPLC-CLS, HPLC-PCR and HPLC-PLS. For comparison purposes, a new validated developed classic HPLC method was used to confirm the results obtained from HPLC-chemometric calibration techniques. A good chromatographic separation between the two drugs with ketoprofen as an internal standard (IS) was achieved using a C18 column 5 Ã?µm, 4.6Ã?â??250 mm and a mobile phase containing 20 mM acetate buffer (pH=3) and methanol [v/v, 25: 75] at flow rate 1.8 ml/ min. Run time was found to be less than 5 minutes. The classic HPLC method was carried out at 255 nm. The developed methods were applied to the quantitative analysis of the investigated drugs in their synthetic mixtures and in tablets. The developed HPLC-chemometric techniques overcome the main problems of the classic HPLC method such as separation treatment, the optimization of experimental conditions and selection of one specific wavelength. HPLC-chemometric techniques using DAD do not require tedious validation steps and eliminate the errors of single regression equations based on single wavelength and provide reliable results with high sensitivity, accuracy and robustness as well as high purity assessment via DAD empowered by PCR and PLS.
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