Without resolving mixture of Ciprofloxacin and Phenylephrine Hydrochloride, simultaneous estimation has been successfully achieved by spectrophotometry. First method, simultaneous equations method employed formation and solving of mathematical simultaneous equations using 272 nm and 238 nm as the λmax of Ciprofloxacin and Phenylephrine Hydrochloride respectively in 0.1 M NaOH. Second method is first order derivative spectroscopy; wavelengths selected for quantitation were 262 nm for Ciprofloxacin (zero crossing point for Phenylephrine Hydrochloride) and 296 nm for Phenylephrine Hydrochloride (zero crossing point for Ciprofloxacin). These methods were validated as per ICH guidelines. Calibration curves were linear over the concentration ranges of 1-12 μg/ml and 5-30 μg/ml for Ciprofloxacin and Phenylephrine Hydrochloride respectively for both the methods. The validation study was statistically significant as all the statistical parameters were within the acceptance range (% RSD < 2.0 and S.D. < 2.0) for both accuracy and precision. The methods are successfully applied to pharmaceutical formulation, with no interference from excipients as indicated by the recovery study. The proposed methods are simple, rapid, economic and accurate for routine simultaneous estimation of Ciprofloxacin and Phenylephrine hydrochloride.
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