Simultaneous spectrophotometric methods are described for the determination of losartan potassium, hydrochlorothiazide and amlodipine besylate in tablets using absorbance correction-H-Point Standard addition method (HPSAM) and partial least squares regression (PLS). A simple and novel method absorbance correction–HPSAM is reported for simultaneous estimation of three drugs using uv-visible spectrophotometry without any prior separation of samples. PLS regression can allow simultaneous spectrophotometric determination of several elements as well as improve the data handling process of complex chemical systems. The linear range was 1-5 µg/ml for losartan and hydrocholrothiazide and 2-10 µg/ml for amlodipine besylate. The relative standard deviation (RSD) for the simultaneous determination of 3 µg/ml of losartan, hydrochorothiazide and amlodipine by applying HPSAM was 1.249, 0.631 and 0.829 respectively. The total relative standard error for applying PLS method to 10 synthetic samples in the linear ranges of these drugs was ranging from 0.018 – 0.045 for all three drugs. The reported methods HPSAM and PLS can be claimed as green analytical chemistry as it does not involve the use of organic solvents and hence can be utilized for the routine analysis of these drugs from tablets.
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