A simple, sensitive, specific and accurate spectrofluorometric method was developed for the simultaneous determination of ebastine and phenylephrine hydrochloride using direct and indirect method. In which phenylephrine hydrochloride gives inherent fluorescence in 0.1 M HCl which was measured at 304.0 nm after excitation at 275.0 nm (direct method) and ebastine is converted to its fluorescence derivative. This involves condensation of the drug with mixed anhydrides (citric and acetic anhydrides) producing a product with intense fluorescence, which was measured at 477 nm after excitation at 376 nm (Indirect method). Linearity was established for ebastine and phenylephrine hydrochloride in the range of 0.2-1.0 μg/ml. The precision of the methods were satisfactory; the values of relative standard deviation did not exceed 2%. This method can be successfully employed for simultaneous quantitative analysis of ebastine and phenylephrine hydrochloride and in bulk drugs and formulations.
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