Simultaneous spectrophotometric determination of Irbesartan (IRB) and Hydrochlorothiazide (HCZ) by H-Point standard addition method (HPSAM) and two chemometric methods Partial least square (PLS-1) and Principle Component regression (PCR) are described. The results of HPSAM showed that simultaneous determination could be performed with the ratio of 1:4 – 6:0.8 for Irbesartan – Hydrochlorothiazide. The chemometric approaches were successfully applied to quantify the two drugs in the mixture using the information included in the UV absorption spectra of appropriate solutions in the range of 200 – 350nm with the intervals = 1 nm at 151 wavelengths. The calibration or regression was obtained by using the absorbance data matrix and concentration data matrix for the prediction of the unknown concentrations of IRB and HCZ in their mixture. The linear range was found to be 1-5 µg/ml and 0.5-2.5 µg/ml in their mixture. The procedure did not require any separation step. The accuracy and precision of the methods were assessed. These methods were successfully applied to a pharmaceutical preparation, tablet and the results were compared with each other.
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