The present cytogenetic investigation was carried out on 16 Purebred Sahiwal cattle maintained at Department of Livestock Production and Management, College of Veterinary Science, Tirupati to know their chromosomal architecture. Short-term lymphocyte culture technique was employed for obtaining the metaphase chromosomes. Chromosomes of thirty well spread metaphases from each animal with a total of 480 metaphase spreads from Purebred Sahiwal cattle were screened for chromosomal count and ten metaphases from each animal were taken for karyotyping. The modal diploid chromosome number (2n) in Sahiwal was found to be 60. All the autosomes were acrocentric and X chromosome was submetacentric and the Y chromosome was acrocentric.
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