Background. Adiponectin (APN) is an adipocyte-derived hormone that has peripheral beneficial effects. Although its receptors\nAdipoR1 and AdipoR2 are expressed in the brain, their function in neurons is poorly understood. The aims of this work were to\ndescribe the distribution of APN receptors in the olfactory bulb (OB) as well as the possible effects of APN injection on the\ninsulin receptor (InsR) content and Akt kinase. Method. We performed the double immunofluorescence technique to describe\nthe distribution of AdipoRs and the cellular type they were expressing. mRNA transcript and protein content were assessed by\nRT-PCR and Western blot, respectively. APN injection was performed to analyze its possible effect on the insulin pathway.\nResults. We found that AdipoRs were localized in all cell layers and in both neurons and astrocytes. We observed the presence\nof mRNA transcripts and immunoblot analysis confirmed the protein on the intact OB; APN injection in the OB resulted in a\nslight decrease of the total InsR and Akt phosphorylation and a reduction of phopho-InsR content. Conclusions. These data\ndemonstrated that AdipoRs are expressed in OB regions, and APN injection could act as an insulin pathway modulator in the\nOB and thus possibly contribute to olfaction physiology.
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