To explore the mechanisms by which andrographolide inhibits gastric cancer cell proliferation and metastasis, we employed the\ngastric cell line SGC7901 to investigate the anticancer effects of andrographolide.The cell survival ratio, cell migration and invasion,\ncell cycle, apoptosis, and matrix metalloproteinase activity were assessed. Moreover, western blotting and real-time PCR were\nused to examine the protein expression levels and the mRNA expression levels, respectively. The survival ratio of cells decreased\nwith an increasing concentration of andrographolide in a dose-dependent manner. Consistent results were also obtained using an\napoptosis assay, as detected by flow cytometry.The cell cycle was blocked at the G2/M2 phase by andrographolide treatment, and\nthe proportion of cells arrested at G1/M was enhanced as the dose increased. Similarly, wound healing and Transwell assays showed\nreduced migration and invasion of the gastric cancer cells at various concentrations of andrographolide. Andrographolide can\ninhibit cell proliferation, invasion, and migration, block the cell cycle, and promote apoptosis in SGC7901 cells. The mechanisms\nmay include upregulated expression of Timp-1/2, cyclin B1, p-Cdc2, Bax, and Bik and downregulated expression of MMP-2/9 and\nantiapoptosis protein Bcl-2.
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