Background. For childhood acute lymphocytic leukemia (ALL), central nervous system leukemia (CNSL) is still themain reason of\ntreatment failure. Changes of cerebrospinal fluid (CSF) proteome are deemed to occur after intrathecal chemotherapy. Objective.\nTo find critical CSF biomarkers, which could be utilized to increase diagnostic and prognostic accuracy of CNSL. Methods. We\nperformed proteomic profiling of CSF before and after the treatment of six sporadic paediatric patients diagnosed as ALL with\ncentral nervous system (CNS) involvement. CSF samples were properly processed and analyzed through the use of label-free liquid\nchromatography-tandem mass spectrometry (LC-MS/MS). Results. Among identified 428 unique proteins in all CSF samples, we\nquantified 10 altered proteins with diverse biological functions after induction chemotherapy. Conclusions. The levels of those 10\nproteins change during the treatment of CNSL. Some of the proteins are likely to play a vital biological role as biomarkers for the\ndevelopment of ALL. In addition, our results indicated the feasible and reproducible utility of CSF for diagnosis and prognosis of\npatients with CNSL.
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