Background. Organ transplantation is considered the best treatment for end-stage organ failure. However, the lack of available\norgans for transplantation and the increasing number of patients waiting for transplants are primary issues facing the transplant\ncommunity.Thus, developing strategies to increase the number of donors, especially for liver transplantation, has become a priority.\nThe use of organs acquired from donors who suffered cardiac related deaths has increased the pool of potential liver donors.\nHowever, donation after cardiac death (DCD) livers increases the risk of primary graft dysfunction. Methods. In the current\nstudy, we conducted transcriptome sequencing using livers from a DCD rat to assess the short-term feasibility and functional\nefficacy of DCD livers. RNA sequencing (RNAseq) data showed that the liver transcriptome varied greatly in rat livers subjected\nto 15 minutes of cardiac arrest. Results. The livers used in the current study had a significant loss of normal function before\ntransplantation. Functional and network analyses consistently indicated that transcription and translation processes were inhibited\nafter approximately 15 minutes of cardiac arrest. Moreover, the transcriptomic sequencing data provides significant insight for\nidentifying functional genes and testing additional biological questions in DCD liver transplantation in future studies.
Loading....