Background: To confirm levels and detection timing of circulating microRNAs (miRNAs)\nin the serum of a mouse model for diagnosis of ototoxicity, circulating miR-205 in the serum was\nevaluated to reflect damages in the cochlear microstructure and compared to a kidney injury model.\nMethod: A microarray for miRNAs in the serum was performed to assess the ototoxic effects\nof kanamycin-furosemide. Changes in the levels for the selected miRNAs (miR-205, miR-183,\nand miR-103) were compared in the serum and microstructures of the cochlea (stria vascularis,\norgan of Corti, and modiolus) between the ototoxicity and normal mouse groups. An acute kidney\ninjury (AKI) mouse model was used to assess changes in miR-205 levels in the kidney by ototoxic\ndrugs. Results: In the mouse model for ototoxicity, the serum levels of circulating miR-205 peaked on\nday 3 and were sustained from days 7â??14. Furthermore, miR-205 expression was highly expressed\nin the organ of Corti at day 5, continued to be expressed in the modiolus at high levels until day\n14, and was finally also in the stria vascularis. The serum miR-205 in the AKI mice did not change\nsignificantly compared to the normal group. Conclusions Circulating miR-205 from the cochlea, after\nototoxic damage, migrates through the blood vessels to organs, which is then finally found in blood.\nIn conditions of hearing impairment with ototoxic medications, detection of circulating miR-205 in\nthe blood can be used to determine the extent of hearing loss. In the future, inner ear damage can be\nidentified by simply performing a blood test before the hearing impairment due to ototoxic drugs.
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