Death of infants from diarrhoea is a common occurrence in sub-Saharan\nAfrica. This is attributed to unhygienic practices which aid the proliferation\nof diarrhoea-causing microorganisms. Among these microorganisms, Campylobacter\nspecies have been reported as one of the causal agents, Campylobacter\nspp. are human intestinal pathogens of global importance and their\npathogenicity mechanisms are not well understood. This study was designed\nto investigate the molecular characterisation of Campylobacter gotten\nfrom cultural methods in Osun State. Campylobacters isolated were\nbiochemically characterized and biotyped. Confirmation of Campylobacter\nwas done using flaA gene, hippuricase O for Campylobacter jejuni and aspartokinase\ngene for Campylobacter coli and single locus sequencing glnA\ngene were performed by PCR. Twenty five samples were amplified by PCR\nout of 57 Campylobacter strains that were positive for cultural methods\nfrom 815 stool samples with diarrhoea and 100 stool samples without diarrhoea.\nNo Campylobacter was isolated from stools of children in the control\ngroup. Twenty-five isolates comprising of 18 Campylobater jejuni and 7 C.\ncoli were identified. The nucleotide sequence of the gln A for all the isolated\nCampylobacter spp. showed 91.0% similarity with the ones in the GenBank.\nThe C. jejuni was classified into biotypes I (44.4%) and II (55.6%) and all C.\ncoli were of biotype I.
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