Introduction. Xeroderma pigmentosum group C (XPC), essential component ofmultisubunit stem cell coactivator complex (SCC),\nfunctions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its\nspecific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown.\nMethods. To elucidate the functional role XPC played in pluripotency andmultilineage differentiation of DPCs, expressions of XPC\ninDPCs with long-term culture were examined by real-time PCR and western blot.DPCs were transfected with lentiviral-mediated\nhuman XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation,\nsenescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC,\nOct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated\nin DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase\nactivity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated,\nand multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an\nessential role in themodulation of pluripotency andmultilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.
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