Background: Reference genes are usually stably expressed in various cells and tissues. However, it was reported\nthat the expression of some reference genes may be distinct in different species. In this study, we intend to answer\nwhether the expression of reported traditional reference genes changes or not in the polyploid fish\nResults: By retrieving the mRNA sequencing data of three different ploidy fish from the NCBI SRA database, we\nselected 12 candidate reference genes, and examined their expression levels in the 10 tissues and in the four cell\nlines of three different ploidy fish by real-time PCR. Then, the expression profiles of these 12 candidate reference\ngenes were systematically evaluated by using the software platforms: BestKeeper, NormFinder and geNorm.\nConclusion: The 28S ribosomal protein S5 gene (RPS5) and the ribosomal protein S18 gene (RPS18) are the most\nsuitable reference genes for the polyploid of Cyprinus carpio and Carassius auratus, demonstrated by both of the\ntissues and the cultured cells.
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