Tuberculosis is a lethal epidemic, difficult to control disease, claiming thousands of lives every year. We have developed a\nnanodelivery formulation based on para-aminosalicylic acid (PAS) and zinc layered hydroxide using zinc nitrate salt as a precursor.\nThe developed formulation has a fourfold higher efficacy of PAS against mycobacterium tuberculosis with a minimum inhibitory\nconcentration (MIC) found to be at 1.40 ????g/mL compared to the free drug PAS with a MIC of 5.0 ????g/mL. The newly developed\nformulation was also found active against Gram-positive bacteria, Gram-negative bacteria, and Candida albicans.The formulation\nwas also found to be biocompatible with human normal lung cells MRC-5 and mouse fibroblast cells-3T3. The in vitro release of\nPAS from the formulation was found to be sustained in a human body simulated phosphate buffer saline (PBS) solution at pH\nvalues of 7.4 and 4.8. Most importantly the nanocomposite prepared using zinc nitrate salt was advantageous in terms of yield and\nfree from toxic zinc oxide contamination and had higher biocompatibility compared to one prepared using a zinc oxide precursor.\nIn summary, these promising in vitro results are highly encouraging for the continued investigation of para-aminosalicylic acid and\nzinc layered hydroxide nanocomposites in vivo and eventual preclinical studies.
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