Glioblastoma multiforme (GBM) is recognized as the most common and lethal form of central nervous system cancer. To cure\nGBM patients, many target-specific chemotherapeutic agents have been developing. However, 2D monolayer cell-based toxicity\nand efficacy tests did not efficiently screen agents due to the pool reflection of in vivo microenvironments (cell-to-cell and cellto-\nextracellular matrix interaction). In this study, we used a 3D cell-based, high-throughput screening method reflecting the\nmicroenvironments using a micropillar and microwell chip platform to draw a high-dose heat map of the cytotoxicity and efficacy\nof 70 compounds, with two DMSO controls.Moreover, the high-dose heatmap model compared the responses of four 3D-cultured\npatient-derived GBM cells and astrocytes to high dosages of compounds with respect to efficacy and cytotoxicity, respectively, to\ndiscern the most efficacious drug for GBM. Among the 70 compounds tested, cediranib (a potent inhibitor of vascular endothelial\ngrowth factor (VEGF) receptor tyrosine kinases) exhibited the lowest cytotoxicity to astrocytes and high efficacy to GBM cells in a\nhigh-dose heat map model.
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