Qinggan Huoxue Recipe is a traditional Chinese medicine, which has been usually used to improve liver function in hepatitis. In\r\norder to investigate the effects of high-dose Qinggan Huoxue Recipe on acute liver failure and explore the potential mechanism, we\r\nhad built acute liver failure models in rats by intraperitoneal injection of D-galactosamine (D-GalN). High-dose Qinggan Huoxue\r\nRecipe was delivered by gavage. After treatment, the blood alanine aminotransferase (ALT), aspartate aminotransferase (AST), total\r\nbilirubin (TBIL), albumin (ALB), cholinesterase (CHE), and prothrombin time (PT) were determined.The pathological score of\r\nliver tissue was recorded. Proliferating cell nuclear antigen (PCNA) immunohistochemistry staining and fluorescence quantitative\r\nreverse transcription polymerase chain reaction (qRT-PCR) of high mobility group box 1 (HMGB1), toll-like receptor 4 (TLR4),\r\nnuclear factor-kappa B (NF-?B), and Caspase-3 were performed.The survival curve was also depicted. Our results demonstrated\r\nthat high-dose QingganHuoxue Recipe could significantly improve liver function and increase survival rates in rats with acute liver\r\nfailure. These effects were supposed to be mediated by suppressing inflammatory reaction and apoptosis.
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