Resealed erythrocyte loaded antiviral agent have potential effective therapy against modified tuberculosis pathogens. The advantages over the administration of rifampicin, includes less dose, decreased toxicity and lower systemic dose which can produce sustained delivery of the rifampicin at peak concentration to the intracellular site of microbial replication and increased therapeutic efficacy. In this research, the entrapment of Rifampicin by rat’s erythrocyte prepared using dilutional haemolysis and preswell dilutional haemolysis methods were studied. The effect of drug concentration and methods of encapsulation on the entrapment efficiency of rifampicin were determined. The hematologic parameters and osmotic fragility of rifampicin loaded erythrocyte carriers were measured. The entrapment of rifampicin by erythrocyte carriers was dependent on the method of encapsulation and amount of drug used. Difference in the osmatic fragility profile between native and drug loaded erythrocytes were observed, which were dependent on method of drug loaded. Mean hematological parameters were evaluated and compared with native erythrocytes. The mean corpuscular volume (MCV) of rifampicin loaded erythrocyte was significantly similar with native forms. The cell recovery of drug loaded erythrocytes were small than native cells. Rifampicin proved a sustained release from loaded erythrocytes over 48 h periods, which suggested an active use of the erythrocyte as a slow systemic release for treatment of tuberculosis infections.
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