Punarnavashtak (PN) kwath formulation mentioned in ayurvedic literature “Bhaishyajyaratnavali”, was evaluated for hepatoprotective potential by its in vivo (Paracetamol induced hepatotoxicity) and clinical study to rationalize the traditional use of this formulation for hepatic disorders. Materials and methods: PN kwath was prepared in the laboratory according to the method given in Ayurvedic literature and phytochemical screening was performed to determine the presence of phytoconstituents. Hepatoprotective and antioxidant activity was evaluated against paracetamol induced hepatotoxicity in rats. For clinical study patients with evidence of liver disease attending outpatient clinics were included in a study and underwent the clinical examination and laboratory investigations for liver function tests before commencement of therapy and after eight weeks of treatment with PN kwath. Results: Preliminary phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, saponins and bitter principles in PN kwath. Administration of PN kwath produced significant hepatoprotective effect as demonstrated by decreased level of serum liver marker enzymes such as AST, ALT, ALP, SBRN and an increased protein level as compared to paracetamol treated group. It also showed antioxidant activity by increase in activity of GSH, SOD, CAT and decrease in TBARS level as compared to paracetamol treated group. A comparative histopathological study of liver exhibited almost normal architecture, as compared to paracetamol treated group. Results of clinical study also establish its hepatoprotective potential. Discussion and conclusion: Results from the current study, establishes a good correlation between in vivo and clinical hepatoprotective activity of PN kwath.
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